Journal: BMC Musculoskeletal Disorders

Article Title: Anti-signal recognition particle positive necrotizing myopathy-sjogren’s syndrome overlap syndrome: a descriptive study on clinical and myopathology features

doi: 10.1186/s12891-023-06354-5

Figure Lengend Snippet: Characteristics of pathological from anti-SRP IMNM overlap SS ( a , c , e , g , i , k , m ) and anti-SRP IMNM patients ( b , d , f , h , j , l , n ). Inflammatory cell infiltrates to the endomysium (E), necrotic(N), perimysial (PM), perivascular (PV) areas. a , b Representative images of H&E stainings revealed remarkable myofibers necrosis and regenerative in both groups. c , d , e , f Representative images of CD4 + and CD68 + of both groups. o , p The numbers of anti-SRP IMNM overlap SS patients were significantly increased in the presence of CD4 + cells in both necrotic( p = 0.023) and endomysial areas ( p = 0.013), as well as in the presence of CD68 + cells ( p = 0.016) infiltrated the endomysial area. g , h , s Representative images of sarcolemmal MAC deposition on both groups. Deposition was more commonly seen in anti-SRP IMNM overlap SS patients ( p = 0.013). i - n Representative images of CD8 + , CD20 + cells and MHC class I upregulation. q , r , t No significant differences were found between two groups. a - b scale bar is 250um, (c-n) scale bar is 100um. Data are given as mean with SE. * P < 0.05. HPF, high-power fields

Article Snippet: The immunological characteristics and inflammatory infiltrates were performed by immunohistochemical staining using anti-CD20 (1:50, A4893, Abclonal), anti-CD4 (1:100, A19018, Abclonal) and anti-CD8 (1:4000, 66,968–1-Ig, Proteintech), anti-CD68 (1:100, sc-20060, Santa), anti-Major Histocompatibility Complex class I (MHC class I) (1:400, ab22432, Abcam), anti-C5b-9 (Membrane attack complex; MAC) antibody (1:200, sc-58935, Santa), anti-CD23 (1:100, 60,208–1-Ig, Proteintech), Activation-induced cytidine deaminase (AID) (1:200, YT5566, North American ImmunoWay Biotechnology Company).

Techniques:

Journal: BMC Musculoskeletal Disorders

Article Title: Anti-signal recognition particle positive necrotizing myopathy-sjogren’s syndrome overlap syndrome: a descriptive study on clinical and myopathology features

doi: 10.1186/s12891-023-06354-5

Figure Lengend Snippet: Pathological manifestations of patients in this study

Article Snippet: The immunological characteristics and inflammatory infiltrates were performed by immunohistochemical staining using anti-CD20 (1:50, A4893, Abclonal), anti-CD4 (1:100, A19018, Abclonal) and anti-CD8 (1:4000, 66,968–1-Ig, Proteintech), anti-CD68 (1:100, sc-20060, Santa), anti-Major Histocompatibility Complex class I (MHC class I) (1:400, ab22432, Abcam), anti-C5b-9 (Membrane attack complex; MAC) antibody (1:200, sc-58935, Santa), anti-CD23 (1:100, 60,208–1-Ig, Proteintech), Activation-induced cytidine deaminase (AID) (1:200, YT5566, North American ImmunoWay Biotechnology Company).

Techniques: Cell Counting, Staining

Journal: Cell Reports Methods

Article Title: Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry

doi: 10.1016/j.crmeth.2021.100104

Figure Lengend Snippet: List of antibody clones validated for FFPE tissue staining with ChipCytometry.

Article Snippet: CD68 , FITC , FS488 , KP1 , mouse , Santa Cruz , sc-20060 FITC , 1:100 , o.n. 4°C , surface.

Techniques: Clone Assay, Staining, Incubation

Journal: Cell Reports Methods

Article Title: Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry

doi: 10.1016/j.crmeth.2021.100104

Figure Lengend Snippet:

Article Snippet: CD68 , FITC , FS488 , KP1 , mouse , Santa Cruz , sc-20060 FITC , 1:100 , o.n. 4°C , surface.

Techniques: Recombinant, Software, Fluorescence